The stilbene disulfonic acid DIDS stimulates the production of TNF-alpha in human lymphocytes
Qian C., Díez J., Larrea E., Garciandía A., Arrazola A., Civeira M.P., Medina J.F., Prieto J.
Department of Internal Medicine, University of Navarra, Pamplona, Spain.
Exposure of human peripheral blood mononuclear cells (PBMC) to the stilbene derivative DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid) (60 microM and above) significantly increased the release of tumor necrosis factor-alpha (TNF-alpha), as determined by TNF-alpha activity in the incubation media.
When the TNF-alpha message was analyzed in PBMC by a reverse transcription/polymerase chain reaction (RT/PCR)-based procedure, it was found that incubation with DIDS (60 microM) was followed by a time-dependent accumulation of TNF-alpha mRNA. Measurements of intracellular pH showed that the presence of increasing concentrations of DIDS resulted in a progressive intracellular alkalinization of PBMC.
It is suggested that the known DIDS effect of inhibiting transmembrane anion exchange, i.e., chloride/bicarbonate exchange, might play a role in the stimulation of TNF-alpha production by PBMC exposed to DIDS.
CITATION Biochem Biophys Res Commun. 1992 Dec 30;189(3):1268-74