Scientific publications 

The increase in fiber size in male rat gastrocnemius after chronic central leptin infusion is related to activation of insulin signaling

Burgos-Ramos E (1), Canelles S (2), Rodríguez A (3), Frago LM (4), Gómez-Ambrosi J (3), Chowen JA 2, Frühbeck G (3), Argente J (5), Barrios V (6).

(1) Department of Endocrinology, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación La Princesa, E-28009, Madrid, Spain; Área de Bioquímica, Facultad de Ciencias Ambientales y Bioquímica, Universidad Castilla-La Mancha, E-45071, Toledo, Spain.
(2) Department of Endocrinology, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación La Princesa, E-28009, Madrid, Spain; Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, E-28009, Madrid, Spain.
(3) Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, E-28009, Madrid, Spain; Metabolic Research Laboratory, Clínica Universidad de Navarra, E-31008, Pamplona, Spain.
(4) Department of Endocrinology, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación La Princesa, E-28009, Madrid, Spain; Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, E-28009, Madrid, Spain; Department of Pediatrics, Universidad Autónoma de Madrid, E-28009, Madrid, Spain.
(5) Department of Endocrinology, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación La Princesa, E-28009, Madrid, Spain; Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, E-28009, Madrid, Spain; Department of Pediatrics, Universidad Autónoma de Madrid, E-28009, Madrid, Spain; IMDEA Food Institute, CEI UAM + CSIC, E-28049, Madrid, Spain.
(6) Department of Endocrinology, Hospital Infantil Universitario Niño Jesús, Instituto de Investigación La Princesa, E-28009, Madrid, Spain; Centro de Investigación Biomédica en Red de Fisiopatología de la Obesidad y Nutrición (CIBEROBN), Instituto de Salud Carlos III, E-28009, Madrid, Spain.

Magazine: Molecular Cell Endocrinology

Date: Jul 15, 2018

Endocrinology and Nutrition [SP]

ABSTRACT

Insulin potentiates leptin effects on muscle accrual and glucose homeostasis. However, the relationship between leptin's central effects on peripheral insulin sensitivity and the associated structural changes remain unclear.

We hypothesized that central leptin infusion modifies muscle size through activation of insulin signaling. Muscle insulin signaling, enzymes of fatty acid metabolism, mitochondrial respiratory chain complexes, proliferating cell nuclear antigen (PCNA) and fiber area were analyzed in the gastrocnemius of chronic central infused (L), pair-fed (PF) and control rats. PCNA-positive nuclei, fiber area, GLUT4 and glycogen levels and activation of Akt and mechanistic target of rapamycin were increased in L, with no changes in PF. Acetyl-CoA carboxylase-β mRNA levels and non-esterified fatty acid and triglyceride content were reduced and carnitine palmitoyltransferase-1b expression and mitochondrial complexes augmented in L.

These results suggest that leptin promotes an increase in muscle size associated with improved insulin signaling favored by lipid profile.

CITATION  Mol Cell Endocrinol. 2018 Jul 15;470:48-59. doi: 10.1016/j.mce.2017.09.029. Epub 2017 Sep 27

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