Scientific publications

Serum and gene expression levels of CT-1, IL-6, and TNF-α after a lifestyle intervention in obese children

Jul 27, 2017 | Magazine: Pediatric Diabetes

Marti A (1,2,3), Morell-Azanza L (1,2), Rendo-Urteaga T (4), García-Calzón S (2,5), Ojeda-Rodríguez A (1,2), Martín-Calvo N (2,3,6), Moreno-Aliaga MJ (1,2,3,7), Martínez JA (1,2,3,7,8), Azcona-San Julián MC (2,9). (1) Department of Nutrition, Food Sciences and Physiology, University of Navarra, Pamplona, Spain.
(2) IdiSNA (Navarra Institute for Health Research), Pamplona, Spain.
(3) Center of Biomedical Research in Physiopathology of Obesity and Nutrition (CIBEROBN), Institute of Health Carlos III, Madrid, Spain.
(4) Youth/Child and cAdiovascular Risk and Environmental (YCARE) Research Group, School of Medicine, University of Sao Paulo, Sao Paulo, Brazil.
(5) Epigenetics and Diabetes Unit, Department of Clinical Sciences, Lund University Diabetes Centre, CRC, Lund University, Malmö, Sweden.
(6) Department of Preventive Medicine & Public Health, School of Medicine, University of Navarra, Pamplona, Spain.
(7) Center for Nutrition Research, University of Navarra, Pamplona, Spain.
(8) Madrid Institute of Advanced Science (IMDEA Food), Madrid, Spain.
(9) Paediatric Endocrinology Unit, Department of Paediatrics, Clinica Universidad de Navarra, Pamplona, Spain.

Inflammation related molecules such as tumor necrosis factor-α (TNF-α), interleukin 6 (IL-6), and cardiotrophin-1 (CT-1) are highly expressed in obese individuals and could partly explain some comorbidities associated to obesity.

In obese children, lifestyle interventions are able to lower inflammation and reduce cardiovascular risk factors associated with obesity.

The aim of the present work was to study changes in inflammation-related molecules serum and peripheral blood mononuclear cells (PBMC) transcript levels after a 10-week lifestyle intervention in obese children and asses their potential association with glucose metabolism.

Twenty-three obese children (mean age 11.5 years; 48% males) underwent a 10-week lifestyle not controlled intervention trial.

Anthropometric and biochemical measurements were analyzed. Transcript analysis for CT-1, IL-6, and TNF-α in PBMC were performed by RT-PCR. Serum cytokine levels were also measured at baseline and after 10-weeks.

Participants achieved a significant reduction in body adiposity (0.34 decrease in body mass index-standard deviation), total cholesterol, and glucose levels after 10-weeks.

A Significant decrease in serum TNF-α and C reactive protein (CRP) were observed. CT-1 transcript levels were significantly reduced (P = .005) after lifestyle intervention, and these changes were significantly correlated with changes in serum CT-1 levels (r = 0.451; P = .031). In multiple regression analysis baseline CT-1 transcript levels were positively associated with final insulin (R2 = 0.506; P = .035) and HOMA-IR values (R2 = 0.473; P = .034).

We reported that serum CRP, TNF-α, as well as PBMC CT-1 transcript levels were reduced after lifestyle intervention in obese children.

More studies are needed to clarify the role of inflammation-related molecules in glucose metabolism.

CITA DEL ARTÍCULO  Pediatr Diabetes. 2017 Jul 27. doi: 10.1111/pedi.12561