Rapid liquid chromatographic assay for the determination of acetaminophen in plasma after propacetamol administration: application to pharmacokinetic studies
Campanero MA, Calahorra B, García-Quétglas E, López-Ocáriz A, Honorato J.
Servicio de Farmacología Clínica, Clinica Universitaria de Navarra, Universidad de Navarra, Pamplona, Spain.
A simple method for the rapid estimation of acetaminophen in plasma is described here. p-Propionamidophenol was used as internal standard.
The assay involved a single ethyl acetate extraction and liquid chromatographic analysis at a wavelength of 242 nm using a reversed-phase encapped column, with a mobile phase of acetonitrile and 0.005 M potassium dihydrogen phosphate adjusted at pH 3.00. The limit of quantitation of acetaminophen by this method was 0.05 microg ml(-1), only 0.1 ml of the plasma sample was required for the determination. The calibration graph was linear from 0.05 to 100 microg ml(-1). Intra and inter-day precision (CV) did not exceed 8.93%. Mean recoveries of 90.31% with a CV of 1.38% were obtained.
Applicability of the method was demonstrated by a pharmacokinetic study in normal volunteers who received 2 mg propacetamol.
CITATION J Pharm Biomed Anal. 1999 Jun;20(1-2):327-34