Physical exercise remodels visceral adipose tissue and mitochondrial lipid metabolism in rats fed a high-fat diet
Rocha-Rodrigues S (1), Rodríguez A (2), Becerril S (2), Ramírez B (2), Gonçalves IO (1), Beleza J (1), Frühbeck G (2,3), Ascensão A (1), Magalhães J (1).
(1) CIAFEL - Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal.
(2) Metabolic Research Laboratory, Clínica Universidad de Navarra; Obesity & Adipobiology Group, Instituto de Investigación Sanitario de Navarra (IdiSNA); CIBEROBN, Instituto de Salud Carlos III, Pamplona, Spain.
(3) Department of Endocrinology & Nutrition, Clínica Universidad de Navarra, Pamplona, Spain.
We aimed to investigate the effects of two physical exercise models, voluntary physical activity (VPA) and endurance training (ET) as preventive and therapeutic strategies, respectively, on lipid accumulation regulators and mitochondrial content in VAT of rats fed a high-fat diet (HFD).
Sprague Dawley rats (6 wks old, n=60) were assigned into sedentary and VPA groups fed isoenergetic diets: standard (S, 35 kcal% fat) or HFD (71 kcal% fat). The VPA groups had free access to wheel running during the entire protocol. After 9-wks, half of sedentary animals were exercised on a treadmill while maintaining the dietary treatments.
HFD induced no changes in plasma non-esterified fatty acids (NEFA) and glycerol levels and decreased oxidative phosphorylation (OXPHOS) subunit IV and increased truncated/full-length sterol regulatory element-binding transcription factor 1c (SREBP1c) ratio in epididymal white adipose tissue (eWAT).
VPA decreased plasma glycerol levels, aquaglyceroporin 7 (AQP7) and increased subunit I of cytochrome c oxidase (COX) protein, in standard fed animals. Eight-wks of ET decreased body weight, visceral adiposity and adipocyte size and plasma NEFA and glycerol levels, as well as AQP7 protein expression in eWAT.
ET increased fatty acid translocase (FAT/CD36), mitochondrial content of complexes IV and V subunits, mitochondrial biogenesis and dynamic (mitofusins and optic atrophy 1)-related proteins. Moreover, lipogenesis-related markers (SREBP1c and acetyl CoA carboxylase) were reduced after 8-wks of ET.
In conclusion, ET-induced alterations reflect a positive effect on mitochondrial function and the overall VAT metabolism of HFD-induced obese rats.
CITATION Clin Exp Pharmacol Physiol. 2016 Nov 22. doi: 10.1111/1440-1681.12706