Isolation, culture and characterization of adult carotid body-derived cells
Amaya Izal-Azcárate (a), María Gutiérrez-Pérez (b), Iñigo Izal (c), Silvia Belzunegui (a), Waldy San Sebastián (a), Berta López (a), Irene Marcilla (a), Felipe Prósper (b) and Maria-Rosario Luquin (a)
(a)Laboratory of Regenerative Therapy, Department of Neuroscience, Centre for Applied Medical Research, University of Navarra, Pamplona, Spain
(b)Hematology and Cell Therapy and Division of Cancer, Clinica Universitaria and Foundation for Applied Medical Research, University of Navarra, Pamplona, Spain
(c) Orthopaedic Research Laboratory, University of Navarra, Pamplona, Spain
Magazine: Respiratory Physiology & Neurobiology
Date: Jun 1, 2009Neurology [SP] Cell Therapy Area [SP]
Recent studies indicate that carotid body (CB) could be a suitable cell source for cell therapy in Parkinson's disease. We have isolated and successfully expanded in culture as monolayer adult CB-derived cells using a modification of the culture medium employed for bone marrow multipotent adult progenitor cells (MAPCs).
These cells express variable amounts of tyrosine hydroxylase (TH), ?-III tubulin and Sox2. In addition, CB-derived cells showed high expression of Sox2 related to a high rate of proliferation and consistent with an undifferentiated state. Under culture conditions that reduced cell proliferation, Sox2 expression decreased while TH and ?-III tubulin expression was increased. This could indicate that the differentiation of some cells occurs in the culture, thus accounting for a certain neural differentiation potential of CB-derived cells.
CITATION Respir Physiol Neurobiol. 2009 Jun 30;167(2):201-7
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