Identification of Resistance Pathways Specific to Malignancy Using Organoid Models of Pancreatic Cancer
Ponz-Sarvise M (1), Corbo V (2), Tiriac H (3), Engle DD (4), Frese KK (5), Oni TE (6), Hwang CI (3), Öhlund D (7), Chio IIC (8), Baker LA (3), Filippini D (3), Wright K (3), Bapiro TE (9), Huang PS (10), Smith PD (11), Yu KH (12), Jodrell DI (13), Park Y (3), Tuveson DA (14).
(1) Medical Oncology, Clinica Universidad de Navarra, Universidad de Navarra.
(2) Diagnostics and Public Health, ARC-Net Research Centre, University and Hospital Trust of Verona.
(3) Cold Spring Harbor Laboratory.
(4) Regulatory Biology Laboratory, Salk Institute for Biological Studies.
(5) Clinical and Experimental Pharmacology Group, Cancer Research UK Manchester Institute.
(6) Cancer Center, Cold Spring Harbor Laboratory.
(7) Wallenberg Centre for Molecular Medicine, Umeå University.
(8) Genetics and Development, Columbia University.
(9) CRUK Cambridge Institute, University of Cambridge.
(10) G&A, Cygnal Therapeutics.
(11) Oncology Bioscience, AstraZeneca (United Kingdom).
(12) Medicine, Memorial Sloan Kettering Cancer Center.
(13) Cancer Research UK Cambridge Institute, University of Cambridge.
(14) Cold Spring Harbor Laboratory
Magazine: Clinical Cancer Research
Date: Sep 6, 2019Medical Oncology
KRAS is mutated in the majority of pancreatic ductal adenocarcinoma. MAPK and PI3K-AKT are primary KRAS effector pathways, but combined MAPK and PI3K inhibition has not been demonstrated to be clinically effective to date. We explore the resistance mechanisms uniquely employed by malignant cells.
We evaluated the expression and activation of receptor tyrosine kinases in response to combined MEK and AKT inhibition in KPC mice and pancreatic ductal organoids. Additionally, we sought to determine the therapeutic efficacy of targeting resistance pathways induced by MEK and AKT inhibition in order to identify malignant-specific vulnerabilities.
Combined MEK and AKT inhibition modestly extended the survival of KPC mice and increased Egfr and ErbB2 phosphorylation levels. Tumor organoids, but not their normal counterparts, exhibited elevated phosphorylation of ERBB2 and ERBB3 after MEK and AKT blockade. A pan-ERBB inhibitor synergized with MEK and AKT blockade in human PDA organoids, whereas this was not observed for the EGFR inhibitor Erlotinib. Combined MEK and ERBB inhibitor treatment of human organoid orthotopic xenografts was sufficient to cause tumor regression in short-term intervention studies.
Analyses of normal and tumor pancreatic organoids revealed the importance of ERBB activation during MEK and AKT blockade primarily in the malignant cultures. The lack of ERBB hyperactivation in normal organoids suggests a larger therapeutic index. In our models pan-ERBB inhibition was synergistic with dual inhibition of MEK and AKT and the combination of a pan-ERBB inhibitor with MEK antagonists showed the highest activity both in vitro and in vivo.
CITATION Clin Cancer Res. 2019 Sep 6. pii: clincanres.1398.2019. doi: 10.1158/1078-0432.CCR-19-1398
you maybe interested
The Clínica is the greater private hospital with technological equipment of Spain, all in a single center.
The professionals of the Clínica perform continuous research and training, always to the benefit of the patient.
Learn why we are different from other healthcare centers. Quality, speed, comfort and results.