Scientific publications

Generation of a Sprague-Dawley-GFP rat iPS cell line

May 15, 2017 | Magazine: Stem Cell Research

Coppiello G (1), Abizanda G (1), Aguado N (1), Iglesias E (1), Iglesias-Garcia O (2), Lo Nigro A (3), Prosper F (4), Aranguren XL (5). (1) Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
(2) Center of Regenerative Medicine in Barcelona (CMRB), Spain.
(3) Ri.Med Foundation, Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Palermo, Italy.
(4) Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain; Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
(5) Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain


ABSTRACT

We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts.

Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers.

Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells derived from the three germ-layers that maintain the GFP expression.

CITATION  Stem Cell Res. 2017 May;21:47-50. doi: 10.1016/j.scr.2017.03.021. Epub 2017 Mar 31.

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