Generation of a Sprague-Dawley-GFP rat iPS cell line
Coppiello G (1), Abizanda G (1), Aguado N (1), Iglesias E (1), Iglesias-Garcia O (2), Lo Nigro A (3), Prosper F (4), Aranguren XL (5).
We generated a rat iPSC line called ATCi-rSD95 from transgenic Sprague-Dawley GFP fetal fibroblasts.
Established ATCi-rSD95 cells present a normal karyotype, silencing of the transgenes and express pluripotency-associated markers.
Additionally, ATCi-rSD95 cells are able to form teratoma with differentiated cells derived from the three germ-layers that maintain the GFP expression.
CITATION Stem Cell Res. 2017 May;21:47-50. doi: 10.1016/j.scr.2017.03.021. Epub 2017 Mar 31.