Development of a chromatographic method for the determination of saquinavir in plasma samples of HIV patients
Magazine: Biomedical chromatology: BMC
Date: Feb 1, 2002Clinical Research Unit [SP] Clinical Pharmacology [SP]
A simple, sensitive and reproducible high-performance liquid chromatographic method for detecting and quantifying saquinavir in human plasma is described. Verapamil was used as internal standard. The method employes a single liquid-liquid extraction step with tert-butil methyl ether followed by chromatography on a Lichrospher 60 Select B C8 reversed-phase column. Ultraviolet detection was used to identify the compounds of interest.
The quantitation limit of saquinavir was 1 ng/mL and only 0.5 mL of plasma sample was required for the determination. The average saquinavir recoveries over a concentration range of 2.5-500 ng/mL ranged from 86 to 95%. Precision and accuracy did not exceed 5%.
CITATION Biomed Chromatogr. 2002 Feb;16(1):7-12
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