Generation of Macaca fascicularis iPS cell line ATCi-MF1 from adult skin fibroblasts using non-integrative Sendai viruses
Coppiello G (1), Abizanda G (1), Aguado N (1), Iglesias E (1), Arellano-Viera E (1), Rodriguez-Madoz JR (1), Carvajal-Vergara X (1), Prosper F (2), Aranguren XL (3).
We generated ATCi-MF1 induced pluripotent stem (iPS) cell line from Macaca fascicularis adult skin fibroblasts using non-integrative Sendai viruses carrying OCT3/4, KLF4, SOX2 and c-MYC.
Once established, ATCi-MF1 cells present a normal karyotype, are Sendai virus-free and express pluripotency associated markers. Microsatellite markers analysis confirmed the origin of the iPS cells from the parental fibroblasts.
Pluripotency was tested with the in vivo teratoma formation assay. ATCi-MF1 cell line may be a useful primate iPS cell model to test different experimental conditions where the use of human cells can imply ethical issues, as microinjection of pluripotent stem cells in pre-implantational embryos.
CITATION Stem Cell Res. 2017 May;21:1-4. doi: 10.1016/j.scr.2017.03.008. Epub 2017 Mar 16.