O-GlcNAcylated p53 in the liver modulates hepatic glucose production
Maria J Gonzalez-Rellan 1 2 , Marcos F Fondevila # 1 2 , Uxia Fernandez # 1 2 , Amaia Rodríguez 2 3 , Marta Varela-Rey 4 5 , Christelle Veyrat-Durebex 6 7 , Samuel Seoane 1 , Ganeko Bernardo 8 9 , Fernando Lopitz-Otsoa 8 , David Fernández-Ramos 5 8 , Jon Bilbao 8 , Cristina Iglesias 1 , Eva Novoa 1 , Cristina Ameneiro 1 , Ana Senra 1 , Daniel Beiroa 1 , Juan Cuñarro 1 , Maria Dp Chantada-Vazquez 10 , Maria Garcia-Vence 10 , Susana B Bravo 10 , Natalia Da Silva Lima 1 , Begoña Porteiro 1 , Carmen Carneiro 1 , Anxo Vidal 1 , Sulay Tovar 1 , Timo D Müller 11 12 , Johan Ferno 13 , Diana Guallar 1 , Miguel Fidalgo 1 , Guadalupe Sabio 14 , Stephan Herzig 15 , Won Ho Yang 16 , Jin Won Cho 16 , Maria Luz Martinez-Chantar 4 , Roman Perez-Fernandez 1 , Miguel López 1 , Carlos Dieguez 1 , Jose M Mato 6 7 9 , Oscar Millet 6 8 , Roberto Coppari 5 , Ashwin Woodhoo 17 18 19 20 , Gema Fruhbeck 2 3 , Ruben Nogueiras 21 22 23
p53 regulates several signaling pathways to maintain the metabolic homeostasis of cells and modulates the cellular response to stress.
Deficiency or excess of nutrients causes cellular metabolic stress, and we hypothesized that p53 could be linked to glucose maintenance. We show here that upon starvation hepatic p53 is stabilized by O-GlcNAcylation and plays an essential role in the physiological regulation of glucose homeostasis.
More specifically, p53 binds to PCK1 promoter and regulates its transcriptional activation, thereby controlling hepatic glucose production. Mice lacking p53 in the liver show a reduced gluconeogenic response during calorie restriction.
Glucagon, adrenaline and glucocorticoids augment protein levels of p53, and administration of these hormones to p53 deficient human hepatocytes and to liver-specific p53 deficient mice fails to increase glucose levels. Moreover, insulin decreases p53 levels, and over-expression of p53 impairs insulin sensitivity.
Finally, protein levels of p53, as well as genes responsible of O-GlcNAcylation are elevated in the liver of type 2 diabetic patients and positively correlate with glucose and HOMA-IR. Overall these results indicate that the O-GlcNAcylation of p53 plays an unsuspected key role regulating in vivo glucose homeostasis.
CITATION Nat Commun. 2021 Aug 20;12(1):5068. doi: 10.1038/s41467-021-25390-0