Publicaciones científicas

Single-Cell RNA-seq Analysis Reveals a Crucial Role for Collagen Triple Helix Repeat Containing 1 (CTHRC1) Cardiac Fibroblasts after Myocardial Infarction

25-sep-2020 | Revista: Circulation

Adrian Ruiz-Villalba  1 , Juan P Romero  2 , Silvia C Hernandez  3 , Amaia Vilas-Zornoza  2 , Nikolaus Fortelny  4 , Laura Castro-Labrador  2 , Patxi San Martin-Uriz  2 , Erika Lorenzo-Vivas  2 , Paula García-Olloqui  3 , Marcel Palacios  5 , Juan José Gavira  5 , Gorka Bastarrika  6 , Stefan Janssens  7 , Ming Wu  7 , Elena Iglesias  3 , Gloria Abizanda  3 , Xabier Martinez de Morentin  8 , Miren Lasaga  8 , Nuria Planell  8 , Christoph Bock  9 , Diego Alignani  10 , Gema Medal  11 , Beatriz Pelacho  12 , Igor Prudovsky  13 , Yong-Ri Jin  13 , Sergey Ryzhov  13 , Haifeng Yin  13 , David Gomez-Cabrero  8 , Volkhard Lindner  13 , David Lara-Astiaso  2 , Felipe Prósper 12

Background: Cardiac fibroblasts (CF) have a central role in the ventricular remodeling process associated with different types of fibrosis. Recent studies have shown that fibroblasts do not respond homogeneously to heart injury. Due to the limited set of bona fide fibroblast markers, a proper characterization of fibroblast population heterogeneity in response to cardiac damage is still missing.

The purpose of this study was to define the CF heterogeneity during ventricular remodeling and the underlying mechanisms that regulate their function.

Methods: Collagen1α1-GFP+ CF were characterized after myocardial infarction (MI) by single-cell and bulk RNA-seq, ATAC-seq and functional assays. Swine and patient samples were studied using bulk RNA-seq.

Results: We identified and characterized a unique CF subpopulation that emerges after MI in mice. These activated fibroblasts exhibit a clear pro-fibrotic signature, express high levels of Collagen Triple Helix Repeat Containing 1 (Cthrc1) and localize into the scar. Non-canonical TGF-β signaling and different transcription factors including SOX9 are important regulators mediating their response to cardiac injury.

Moreover, the absence of CTHRC1 results in pronounced lethality due to ventricular rupture. Finally, a population of CF with a similar transcriptome was identified in a swine model of MI and in heart tissue from patients with MI and dilated cardiomyopathy.

Conclusions: We report CF heterogeneity, their dynamics during the course of MI and redefine the CF that respond to cardiac injury and participate in myocardial remodeling. Our study identifies Cthrc1 as a novel regulator of the healing scar process, and as a target for future translational studies.

CITA DEL ARTÍCULO  Circulation. 2020 Sep 25.  doi: 10.1161/CIRCULATIONAHA.119.044557