Molecular Imaging Techniques to Study the Biodistribution of Orally Administered (99m)Tc-Labelled Naive and Ligand-Tagged Nanoparticles

PURPOSE
Study by molecular imaging the biodistribution of poly(anhydride) nanoparticles after oral administration.

PROCEDURES
Poly (anhydride) nanoparticles (NP) and cyclodextrin-tagged nanoparticles (CD-NP) were radiolabelled with (99m)Tc. Radiochemical purity was measured with a double-solvent chromatography system and the absence of undesirable components was confirmed by size and polydispersion measurement of the technetium-labelled nanoparticles by photon correlation spectroscopy. Single photon emission computed tomography (SPECT) fused computed tomography (CT) in vivo molecular imaging was used for biodistribution studies in small animals.

RESULTS
SPECT-CT images revealed activity only in the gastrointestinal tract. Thirteen percent of the given dose of CD-NP and 3% of the given dose of conventional NP were found in the stomach at 8 h.

CONCLUSION
No evidence of translocation or distribution out of gastrointestinal tract was found. CD-NP moved significantly more slowly inside the gut than conventional NP, probably due to their physico-chemical structure that allows stronger interactions with the gut mucosa.

CITA DEL ARTÍCULO  Mol Imaging Biol. 2010 Dec 16