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Publicaciones científicas

Generation and characterization of human iPSC line generated from mesenchymal stem cells derived from adipose tissue

01-ene-2016 | Revista: Stem Cell Research

Zapata-Linares N (1), Rodriguez S (1), Mazo M (1), Abizanda G (1), Andreu EJ (2), Barajas M (2), Prosper F (3), Rodriguez-Madoz JR (4).
(1) Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain.
(2) Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain.
(3) Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain; Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain. Electronic address: fprosper@unav.es.
(4) Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Instituto de Investigación Sanitaria de Navarra, Pamplona, Spain.


RESUMEN

In this work, mesenchymal stem cells derived from adipose tissue (ADSCs) were used for the generation of the human-induced pluripotent stem cell line G15.AO. Cell reprogramming was performed using retroviral vectors containing the Yamanaka factors, and the generated G15.AO hiPSC line showed normal karyotype, silencing of the exogenous reprogramming factors, induction of the typical pluripotency-associated markers, alkaline phosphatase enzymatic activity, and in vivo and in vitro differentiation ability to the three germ layers.

CITA DEL ARTÍCULO  Stem Cell Res. 2016 Jan;16(1):20-3. doi: 10.1016/j.scr.2015.12.002. Epub 2015 Dec 2.

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