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Publicaciones científicas

Induction of Cyclooxygenase-2 by Overexpression of the Human NADPH Oxidase 5 (NOX5) Gene in Aortic Endothelial Cells

06-mar-2020 | Revista: Cells

Marqués J (1,2), Cortés A (1,2), Pejenaute Á (1,2), Ansorena E (1,2), Abizanda G (2,3), Prósper F (2,3,4), Martínez-Irujo JJ (1,2), Miguel C (1,2), Zalba G (1,2).

(1) Department of Biochemistry and Genetics, University of Navarra, 31008 Pamplona, Spain.
(2) Navarra Institute for Health Research (IdiSNA), 31008 Pamplona, Spain.
(3) Hematology Service, Clínica Universidad de Navarra, University of Navarra, 31008 Pamplona, Spain.
(4) CIBERONC, 31008 Madrid, Spain.


ABSTRACT

Oxidative stress is a main molecular mechanism that underlies cardiovascular diseases. A close relationship between reactive oxygen species (ROS) derived from NADPH oxidase (NOX) activity and the prostaglandin (PG) biosynthesis pathway has been described.

However, little information is available about the interaction between NOX5 homolog-derived ROS and the PG pathway in the cardiovascular context.

Our main goal was to characterize NOX5-derived ROS effects in PG homeostasis and their potential relevance in cardiovascular pathologies. For that purpose, two experimental systems were employed: an adenoviral NOX5-β overexpression model in immortalized human aortic endothelial cells (TeloHAEC) and a chronic infarction in vivo model developed from a conditional endothelial NOX5 knock-in mouse.

NOX5 increased cyclooxygenase-2 isoform (COX-2) expression and prostaglandin E2 (PGE2) production through nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in TeloHAEC. Protein kinase C (PKC) activation and intracellular calcium level (Ca++) mobilization increased ROS production and NOX5 overexpression, which promoted a COX-2/PGE2 response in vitro.

In the chronic infarction model, mice encoding endothelial NOX5 enhanced the cardiac mRNA expression of COX-2 and PGES, suggesting a COX-2/PGE2 response to NOX5 presence in an ischemic situation. Our data support that NOX5-derived ROS may modulate the COX-2/PGE2 axis in endothelial cells, which might play a relevant role in the pathophysiology of heart infarction.

CITA DEL ARTÍCULO  Cells. 2020 Mar 6;9(3). pii: E637. doi: 10.3390/cells9030637

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