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Publicaciones científicas

Isolation and characterization of Sprague-Dawley and Wistar Kyoto GFP rat embryonic stem cells

15-may-2017 | Revista: Stem Cell Research

Coppiello G (1), Abizanda G (1), Aguado N (1), Iglesias E (1), Iglesias-Garcia O (2), Lo Nigro A (3), Prosper F (4), Aranguren XL (5). (1) Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
(2) Center of Regenerative Medicine in Barcelona (CMRB), Spain.
(3) Ri.Med Foundation, Department of Laboratory Medicine and Advanced Biotechnologies, IRCCS-ISMETT (Istituto Mediterraneo per i Trapianti e Terapie ad Alta Specializzazione), Palermo, Italy.
(4) Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain; Cell Therapy Area, Clínica Universidad de Navarra, University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.
(5) Cell Therapy Program, Center for Applied Medical Research (CIMA), University of Navarra, Health Research Institute of Navarra, Pamplona, Spain.


RESUMEN

We generated two rat embryonic stem cell (ESC) lines: ATCe-SD7.8 from Sprague-Dawley strain and ATCe-WK1 from Wistar Kyoto strain. Cells were marked with enhanced green fluorescent protein (eGFP) by transduction with a lentiviral vector.

Cells present a normal karyotype and express pluripotency-associated markers. Pluripotency was tested in vivo with the teratoma formation assay.

Cells maintain eGFP expression upon differentiation to the three-germ layers. These cells can be a useful tool for cell therapy studies and chimera generation as they can be easily tracked by eGFP expression.

CITA DEL ARTÍCULO  Stem Cell Res. 2017 May;21:40-43. doi: 10.1016/j.scr.2017.03.020. Epub 2017 Mar 29.

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