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Publicaciones científicas

Effects of physical exercise on myokines expression and brown adipose-like phenotype modulation in rats fed a high-fat diet

27-sep-2016 | Revista: Life Sciences

Rocha-Rodrigues S (1), Rodríguez A (2), Gouveia AM (3), Gonçalves IO (4), Becerril S (2), Ramírez B (2), Beleza J (4), Frühbeck G (5), Ascensão A (4), Magalhães J (4).
(1) CIAFEL - Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal.
(2) Metabolic Research Laboratory, Clínica Universidad de Navarra, Pamplona, Spain; Obesity & Adipobiology Group, Instituto de Investigación Sanitario de Navarra (IdiSNA), Pamplona, Spain; CIBEROBN, Instituto de Salud Carlos III, Pamplona, Spain.
(3) Department of Experimental Biology, Faculty of Medicine, University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, Institute for Molecular and Cell Biology (IBMC), University of Porto, Porto, Portugal.
(4) CIAFEL - Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal.
(5) Metabolic Research Laboratory, Clínica Universidad de Navarra, Pamplona, Spain; Department of Endocrinology & Nutrition, Clínica Universidad de Navarra, Pamplona, Spain; Obesity & Adipobiology Group, Instituto de Investigación Sanitario de Navarra (IdiSNA), Pamplona, Spain; CIBEROBN, Instituto de Salud Carlos III, Pamplona, Spain.


AIMS:
Exercise-stimulated myokine secretion into circulation may be related with browning in white adipose tissue (WAT), representing a positive metabolic effect on whole-body fat mass. However, limited information is yet available regarding the impact of exercise on myokine-related modulation of adipocyte phenotype in WAT from obese rats.

MAIN METHODS:
Sprague-Dawley rats (n=60) were divided into sedentary and voluntary physical activity (VPA) groups and fed with standard (35kcal% fat) or high-fat (HFD, 71kcal% fat)-isoenergetic diets.

The VPA-groups had unrestricted access to wheel running throughout the protocol. After-9weeks, half of sedentary standard (SS) and sedentary HFD (HS)-fed animals were exercised on treadmill (endurance training, ET) for 8-weeks while maintaining the dietary treatments.

KEY FINDINGS:
The adipocyte hypertrophy induced by HFD were attenuated by VPA and ET. HFD decreased 5' AMP-activated protein kinase (AMPK) activity in muscle as well as peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) and uncoupling protein 1 (UCP1) proteins in eWAT, while not affecting circulating irisin.

VPA increased eWAT Tmem26 mRNA levels in the standard diet-fed group, whereas ET increased AMPK, interleukin 6 (IL-6) and fibronectin type III domain-containing protein 5 (FNDC5) protein expression in muscle, but had no impact on circulating irisin protein content.

In eWAT, ET increased bone morphogenetic protein 7 (Bmp7), Cidea and PGC-1α in both diet-fed animals, whereas BMP7, Prdm16, UCP1 and FNDC5 only in standard diet-fed group.

SIGNIFICANCE:
Data suggest that ET-induced myokine production seems to contribute, at least in part, to the "brown-like" phenotype in WAT from rats fed a HFD.

CITA DEL ARTÍCULO  Life Sci. 2016 Sep 27. pii: S0024-3205(16)30580-X. doi: 10.1016/j.lfs.2016.09.023