The stilbene disulfonic acid DIDS stimulates the production of TNF-alpha in human lymphocytes
Qian C., Díez J., Larrea E., Garciandía A., Arrazola A., Civeira M.P., Medina J.F., Prieto J.
Department of Internal Medicine, University of Navarra, Pamplona, Spain.
Magazine: Biochemical and Biophysical Research Communications
Date: Dec 1, 1992Internal Medicine [SP]
Exposure of human peripheral blood mononuclear cells (PBMC) to the stilbene derivative DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid) (60 microM and above) significantly increased the release of tumor necrosis factor-alpha (TNF-alpha), as determined by TNF-alpha activity in the incubation media.
When the TNF-alpha message was analyzed in PBMC by a reverse transcription/polymerase chain reaction (RT/PCR)-based procedure, it was found that incubation with DIDS (60 microM) was followed by a time-dependent accumulation of TNF-alpha mRNA. Measurements of intracellular pH showed that the presence of increasing concentrations of DIDS resulted in a progressive intracellular alkalinization of PBMC.
It is suggested that the known DIDS effect of inhibiting transmembrane anion exchange, i.e., chloride/bicarbonate exchange, might play a role in the stimulation of TNF-alpha production by PBMC exposed to DIDS.
CITATION Biochem Biophys Res Commun. 1992 Dec 30;189(3):1268-74
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