Nonsteroidal anti-inflammatory drugs enhance IgE-mediated activation of human basophils in patients with food anaphylaxis dependent and independent of nonsteroidal anti-inflammatory drugs
Pascal M (1,2), Muñoz-Cano R (2,3), Milà J (1), Sanz ML [SP] (4), Diaz-Perales A (5), Sánchez-López J (2,3), García-Moral A (2,3), Juan M (1,2), Valero A (2,3), Yagüe J (1,2), Picado C (2,3), Bartra J (2,3).
(1) Servei d'Immunologia. Centre de Diagnostic Biomedic (CDB), Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.
(2) Institut d'Investigacions Biomediques August Pi i Sunyer (IDIBAPS), Barcelona, Spain.
(3) Unitat d'Al·lergia, Servei de Pneumologia, Hospital Clinic, Universitat de Barcelona, Barcelona, Spain.
(4) Clinica Universitaria de Navarra (CUN), Pamplona, Spain.
(5) Centro de Biotecnologia y Genomica de Plantas, Universidad Politecnica de Madrid-Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria, Madrid, Spain.
Magazine: Clinical Experience Allergy
Date: Mar 28, 2016Allergology and Immunology Department
Nonsteroidal anti-inflammatory drugs (NSAIDs) act as co-factors worsening the allergic reactions induced by food allergens.
To evaluate the effect of both lysine acetylsalicylate (L-ASA) (non-selective cyclooxygenase (COX) inhibitor) and valdecoxib (selective COX-2 inhibitor) in basophils activated by peach Lipid Transfer Protein (Pru p 3) in patients with food-dependent NSAID-induced anaphylaxis (FDNIA).
Twenty Pru p 3 allergic patients with FDNIA group, eleven peach anaphylaxis not exacerbated by NSAIDs (no-NSAIDs group), and 5 healthy volunteers were recruited. Basophil activation (BA) was measured as expression of CD63 (Flow2 CAST™ , Bühlmann® ), after stimulation with Pru p 3, both alone and in combination with L-ASA (1.13, 3.38 and 6.78 mM) or valdecoxib (0.87, 7.8 and 31.25 μM).
Basophils from no-NSAID group were significantly more reactive and sensitive to Pru p 3 than those from the FDNIA group. In both groups, an increase in BA was observed when basophils were exposed to Pru p 3 and L-ASA. In the FDNIA group, valdecoxib partially abrogates the BA induced by Pru p 3, whereas in the no-NSAID group, a dual effect was observed depending on the concentration tested.
This study indicates that subjects with food-induced anaphylaxis differ from FDNIA subjects in the higher reactivity and sensitivity of their basophils to allergen challenge. We have shown a direct effect of NSAIDs on basophils using a human model of FDNIA. Our results also suggest that selective COX2 inhibitors might be a safe alternative. BA test may be a useful tool in the study of the pathogenic mechanism of the cofactor phenomenon.
CITATION Clin Exp Allergy. 2016 Mar 28. doi: 10.1111/cea.12735
you maybe interested
The Clínica is the greater private hospital with technological equipment of Spain, all in a single center.
The professionals of the Clínica perform continuous research and training, always to the benefit of the patient.
Learn why we are different from other healthcare centers. Quality, speed, comfort and results.