Scientific publications
Isolation, culture and characterization of adult carotid body-derived cells
Amaya Izal-Azcárate (a), María Gutiérrez-Pérez (b), Iñigo Izal (c), Silvia Belzunegui (a), Waldy San Sebastián (a), Berta López (a), Irene Marcilla (a), Felipe Prósper (b) and Maria-Rosario Luquin (a)
Recent studies indicate that carotid body (CB) could be a suitable cell source for cell therapy in Parkinson's disease. We have isolated and successfully expanded in culture as monolayer adult CB-derived cells using a modification of the culture medium employed for bone marrow multipotent adult progenitor cells (MAPCs).
These cells express variable amounts of tyrosine hydroxylase (TH), ?-III tubulin and Sox2. In addition, CB-derived cells showed high expression of Sox2 related to a high rate of proliferation and consistent with an undifferentiated state. Under culture conditions that reduced cell proliferation, Sox2 expression decreased while TH and ?-III tubulin expression was increased. This could indicate that the differentiation of some cells occurs in the culture, thus accounting for a certain neural differentiation potential of CB-derived cells.
CITATION Respir Physiol Neurobiol. 2009 Jun 30;167(2):201-7
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