Alpha-MSH regulates interleukin-10 expression by human keratinocytes
Redondo P, García-Foncillas J, Okroujnov I, Bandrés E.
Department of Dermatology, University Clinic of Navarra, School of Medicine, Pamplona, Spain.
Magazine: Archives of Dermatologic Research
Date: Aug 1, 1998Clinical Genetics Unit [SP] Dermatology
Normal human keratinocytes (HKC) are able to synthesize alpha-MSH. Because the production of alpha-MSH by HKC is induced significantly by ultraviolet B radiation, the involvement of keratinocyte-derived alpha-MSH in UV-induced immunosuppression has been suggested.
The induction of the antiinflammatory cytokine IL-10 in monocytes is a major mechanism in the antiinflammatory actions of alpha-MSH. In the present study, HKC were investigated for their ability to produce IL-10 after alpha-MSH stimulation. HKC were obtained from the skin of human female breast sections and either left untreated or treated with 0.01 or 0.1 microg/ml alpha-MSH for different times.
Using RT-PCR, HKC were shown to express IL-10 mRNA even under basal conditions, and treatment with alpha-MSH increased expression. Only minimal concentrations of IL-10 protein were detected in supernatants from the alpha-MSH-stimulated cultures.
To the best of our knowledge this is the first report of IL-10 expression by cultured HKC after alpha-MSH stimulation. Further studies are needed to determine the biological and therapeutic relevance of these findings.
CITATION Arch Dermatol Res. 1998 Aug;290(8):425-8
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