PUBLICAÇÕES científicas

Validation of Endogenous Control Genes in Human Adipose Tissue: Relevance to Obesity and Obesity-associated Type 2 Diabetes Mellitus

V. Catalán [ES] (1), J. Gómez-Ambrosi [ES] (1), F. Rotellar (2), C. Silva [ES] (3), A. Rodríguez (1), J. Salvador (3), M. J. Gil [ES] (4), J. A. Cienfuegos (2), G. Frühbeck (1,3)
(1) Metabolic Research Laboratory, Clínica Universitaria de Navarra, University of Navarra, Pamplona, Spain
(2) Department of Surgery, Clínica Universitaria de Navarra, University of Navarra, Pamplona, Spain
(3) Department of Endocrinology, Clínica Universitaria de Navarra, University of Navarra, Pamplona, Spain
(4) Department of Biochemistry, Clínica Universitaria de Navarra, University of Navarra, Pamplona, Spain

Revisão:Hormone and Metabolic Research

Data: 1/Jul/2007

Cirurgia Geral e Digestiva Bioquímica Clínica [ES] Endocrinologia e Nutrição [ES]

The aim of the present study was to test the influence of obesity and the presence of type 2 diabetes mellitus (T2DM) on the expression of ten housekeeping genes and of the 18S rRNA in a group of human adipose tissue samples from the omental and subcutaneous depot.

Adipose tissue biopsies were obtained by laparoscopic surgery from lean and obese patients. After the extraction, mRNA levels in adipose tissue samples were quantified by real-time PCR using the commercial HUMAN ENDOGENOUS CONTROL PLATES.

From the genes analyzed, 18S rRNA exhibited the most stable expression levels in both depots regardless of the pathophysiological conditions of obesity and obesity-associated T2DM. Contrarily, GAPD was the gene with the highest variation in its expression levels, being upregulated (8.0-fold) in the obese group and downregulated (3.5-fold) in obesity-associated T2DM.

Our results show that 18S rRNA may be the most suitable gene for normalization in expression studies performed in human adipose tissue samples obtained from patients suffering from obesity and/or obesity-associated T2DM, whereas GAPD is less appropriate for comparison purposes under these circumstances.

CITAÇÃO DO ARTIGO  Horm Metab Res. 2007 Jul;39(7):495-500

talvezlhe interesse

QUE TECNOLOGIA UTILIZAMOS? 

A Clínica é o hospital privado com maiores recursos tecnológicos de Espanha, tudo num único centro.

Imagen de un PET, tecnología de vanguardia en la Clínica Universidad de Navarra

OS NOSSOS
PROFISSIONAIS

Os profissionais da Clínica realizam um trabalho contínuo de investigação e formação, sempre em benefício do paciente.

Imagen profesionales de la Clínica Universidad de Navarra

RAZÕES PARA VIR
À CLÍNICA

Conheça porque é que somos diferentes em relação a outros centros sanitários. Qualidade, rapidez, comodidade e resultados.

Imagen del edificio de la Clínica Universidad de Navarra