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Rapid liquid chromatographic assay for the determination of acetaminophen in plasma after propacetamol administration: application to pharmacokinetic studies

Campanero MA, Calahorra B, García-Quétglas E, López-Ocáriz A, Honorato J.
Servicio de Farmacología Clínica, Clinica Universitaria de Navarra, Universidad de Navarra, Pamplona, Spain.

Revisão:Journal of Pharmaceutical and Biomedical Analysis

Data: 1/Jun/1999

Farmacologia Clínica [ES]

RESUMO

A simple method for the rapid estimation of acetaminophen in plasma is described here. p-Propionamidophenol was used as internal standard.

The assay involved a single ethyl acetate extraction and liquid chromatographic analysis at a wavelength of 242 nm using a reversed-phase encapped column, with a mobile phase of acetonitrile and 0.005 M potassium dihydrogen phosphate adjusted at pH 3.00. The limit of quantitation of acetaminophen by this method was 0.05 microg ml(-1), only 0.1 ml of the plasma sample was required for the determination. The calibration graph was linear from 0.05 to 100 microg ml(-1). Intra and inter-day precision (CV) did not exceed 8.93%. Mean recoveries of 90.31% with a CV of 1.38% were obtained.

Applicability of the method was demonstrated by a pharmacokinetic study in normal volunteers who received 2 mg propacetamol.

CITAÇÃO DO ARTIGO  J Pharm Biomed Anal. 1999 Jun;20(1-2):327-34

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