PUBLICAÇÕES científicas

Effects of physical exercise on myokines expression and brown adipose-like phenotype modulation in rats fed a high-fat diet

Rocha-Rodrigues S (1), Rodríguez A [ES] (2), Gouveia AM (3), Gonçalves IO (4), Becerril S (2), Ramírez B (2), Beleza J (4), Frühbeck G (5), Ascensão A (4), Magalhães J (4).
(1) CIAFEL - Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal.
(2) Metabolic Research Laboratory, Clínica Universidad de Navarra, Pamplona, Spain; Obesity & Adipobiology Group, Instituto de Investigación Sanitario de Navarra (IdiSNA), Pamplona, Spain; CIBEROBN, Instituto de Salud Carlos III, Pamplona, Spain.
(3) Department of Experimental Biology, Faculty of Medicine, University of Porto, Porto, Portugal; Instituto de Investigação e Inovação em Saúde, Institute for Molecular and Cell Biology (IBMC), University of Porto, Porto, Portugal.
(4) CIAFEL - Research Centre in Physical Activity, Health and Leisure, Faculty of Sport, University of Porto, Porto, Portugal.
(5) Metabolic Research Laboratory, Clínica Universidad de Navarra, Pamplona, Spain; Department of Endocrinology & Nutrition, Clínica Universidad de Navarra, Pamplona, Spain; Obesity & Adipobiology Group, Instituto de Investigación Sanitario de Navarra (IdiSNA), Pamplona, Spain; CIBEROBN, Instituto de Salud Carlos III, Pamplona, Spain.

Revisão:Life Sciences

Data: 27/Set/2016

Endocrinologia e Nutrição [ES]

AIMS:
Exercise-stimulated myokine secretion into circulation may be related with browning in white adipose tissue (WAT), representing a positive metabolic effect on whole-body fat mass. However, limited information is yet available regarding the impact of exercise on myokine-related modulation of adipocyte phenotype in WAT from obese rats.

MAIN METHODS:
Sprague-Dawley rats (n=60) were divided into sedentary and voluntary physical activity (VPA) groups and fed with standard (35kcal% fat) or high-fat (HFD, 71kcal% fat)-isoenergetic diets.

The VPA-groups had unrestricted access to wheel running throughout the protocol. After-9weeks, half of sedentary standard (SS) and sedentary HFD (HS)-fed animals were exercised on treadmill (endurance training, ET) for 8-weeks while maintaining the dietary treatments.

KEY FINDINGS:
The adipocyte hypertrophy induced by HFD were attenuated by VPA and ET. HFD decreased 5' AMP-activated protein kinase (AMPK) activity in muscle as well as peroxisome proliferator-activated receptor gamma coactivator-1α (PGC-1α) and uncoupling protein 1 (UCP1) proteins in eWAT, while not affecting circulating irisin.

VPA increased eWAT Tmem26 mRNA levels in the standard diet-fed group, whereas ET increased AMPK, interleukin 6 (IL-6) and fibronectin type III domain-containing protein 5 (FNDC5) protein expression in muscle, but had no impact on circulating irisin protein content.

In eWAT, ET increased bone morphogenetic protein 7 (Bmp7), Cidea and PGC-1α in both diet-fed animals, whereas BMP7, Prdm16, UCP1 and FNDC5 only in standard diet-fed group.

SIGNIFICANCE:
Data suggest that ET-induced myokine production seems to contribute, at least in part, to the "brown-like" phenotype in WAT from rats fed a HFD.

CITAÇÃO DO ARTIGO  Life Sci. 2016 Sep 27. pii: S0024-3205(16)30580-X. doi: 10.1016/j.lfs.2016.09.023

talvezlhe interesse

QUE TECNOLOGIA UTILIZAMOS? 

A Clínica é o hospital privado com maiores recursos tecnológicos de Espanha, tudo num único centro.

Imagen de un PET, tecnología de vanguardia en la Clínica Universidad de Navarra

OS NOSSOS
PROFISSIONAIS

Os profissionais da Clínica realizam um trabalho contínuo de investigação e formação, sempre em benefício do paciente.

Imagen profesionales de la Clínica Universidad de Navarra

RAZÕES PARA VIR
À CLÍNICA

Conheça porque é que somos diferentes em relação a outros centros sanitários. Qualidade, rapidez, comodidade e resultados.

Imagen del edificio de la Clínica Universidad de Navarra