Bioadhesive properties and biodistribution of cyclodextrin-poly(anhydride) nanoparticles
Agüeros M, Areses P, Campanero MA, Salman H, Quincoces G [ES], Peñuelas I [ES], Irache JM.
Department of Pharmacy and Pharmaceutical Technology, University of Navarra, Apartado 177, 31008 Pamplona, Spain.
Revisão:European Journal of Pharmaceutical Sciences
Data: 28/Jun/2009Medicina Nuclear [ES] Unidad de Radiofarmacia [ES] Unidade de Investigação Clínica [ES]
This work describes the preparation, characterization and evaluation of the nanoparticles formed by the copolymer of methyl vinyl ether and maleic anhydride (Gantrez) AN) and cyclodextrins, including beta-cyclodextrin (CD) hydroxypropyl-beta-cyclodextrin (HPCD) and 6-monodeoxy-6-monoamino-beta-cyclodextrin (NHCD).
The cyclodextrin-poly(anhydride) nanoparticles were prepared by a solvent displacement method and characterized by measuring the size, zeta potential, morphology and composition.
For bioadhesion studies, nanoparticles were fluorescently labelled with rhodamine B isothiocianate (RBITC). For in vivo imaging biodistribution studies, (99m)Tc-labelled nanoparticles were used. Nanoparticles displayed a size of about 150nm and a cyclodextrin content which was found optimal under the following experimental conditions: cyclodextrin/poly(anhydride) ratio of 0.25 by weight, 30min of incubation time between the cyclodextrin and the polymer. Moreover, the oligosaccharide content was higher with CD than with NHCD and HPCD.
Overall, cyclodextrin-poly(anhydride) nanoparticles displayed homogeneous bioadhesive interactions within the gut. The intensity of these interactions was higher than for control nanoparticles.
The high bioadhesive capacity was observed for HPCD-NP and NHCD-NP which can be related with their rough morphology and, thus, a higher specific surface than for smooth nanoparticles (CD-NP). Finally, from in vivo studies, no evidence of translocation of distribution to other organs was observed when these nanoparticles were orally administered.R
CITAÇÃO DO ARTIGO Eur J Pharm Sci. 2009 Jun 28;37(3-4):231-40
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