A sensitive method for the determination of gemfibrozil in human plasma samples by RP-LC
González-Peñas E, Agarraberes S, López-Ocariz A, García-Quetglas E, Campanero MA, Carballal JJ, Honorato J.
Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad de Navarra, 31008 Pamplona, Spain.
Revisão:Journal of Pharmaceutical and Biomedical Analysis
Data: 1/Ago/2001Farmacologia Clínica [ES]
A sensitive high-performance liquid chromatographic assay for the quantitative determination of gemfibrozil is described in this work. Ibuprofen was used as internal standard. The assay involved a single cyclohexane extraction and LC analysis with fluorescence detection. Chromatography was performed at 40 degrees C on a Hypersil ODS column. The mobile phase was a mixture of a solution of phosphoric acid 0.4% and acetonitrile (45:55). The method was validated.
The detection limit of this method was 0.025 microg ml(-1); only 0.5 ml of the plasma sample was required for the determination. The calibration graph was linear from 0.05 to 0.5 microg ml(-1) and required a cubic equation from 0.5 to 30 microg ml(-1). Intra and inter-day precision (C.V.) did no exceed 15%.
Mean recoveries were of 90.15+/-6.9% (C.V.'s<8%) for gemfibrozil and 93.10% for ibuprofen Applicability of the method was demonstrated by a pharmacokinetic study in normal volunteers who received gemfibrozil by oral route.
CITAÇÃO DO ARTIGO J Pharm Biomed Anal. 2001 Aug;26(1):7-14
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