Publicaciones científicas

Comparison of ex vivo expansion culture conditions of mesenchymal stem cells for human cell therapy

Maitane Pérez-Ilzarbe,* María Díez-Campelo,* Pablo Aranda, Soraya Tabera, Tania Lopez, Consuelo del Cañizo, Juana Merino, Cristina Moreno, Enrique J. Andreu, Felipe Prósper, and José Antonio Pérez-Simón
*Hematology, Cell Therapy Area and Immunology Service, Clínica Universitaria, Universidad de Navarra, Pamplona; the Department of Hematology, Hospital Clínico Universitario de Salamanca, Salamanca; and the Centro de Medicina Regenerativa y Terapia Celular de Castilla y Leon, Castilla y Leon, Spain.

Revista: Transfusion

Fecha: 20-may-2009

Inmunología e Inmunoterapia Área de Terapia Celular

BACKGROUND
Mesenchymal stem cells (MSCs) are multipotent stem cells. Based on their properties, several clinical trials have been designed to explore their potential therapeutic effect. Fetal calf serum (FCS, commonly used for in vitro expansion) is an undesirable source of xenogeneic antigens and bears the risk of transmitting contaminations. As an alternative for FCS, platelet lysate (PL) and both autologous and allogeneic human serum have been proposed.

The aim of this study is to compare the culture of bone marrow (BM)- derived MSCs in the presence of different serum supplements to determine the effect on cell growth, differentiation potential, and immunologic function.

STUDY DESIGN AND METHODS
MSCs from BM of healthy volunteer donors were grown in the presence of 10% FCS supplemented with 1 ng/mL basic fibroblast growth factor (bFGF), 10% human serum supplemented with 1 ng/mL bFGF, 5% PL, and PL 5% supplemented with 1 ng/mL bFGF (PL plus bFGF).

RESULTS
MSCs that expanded in either medium showed a comparable morphology, phenotype, and proliferative and differentiation capacity. While the presence of MSCs in vitro significantly decreased CD3/ CD28-mediated T-cell activation, this effect was significantly higher in MSCs cultured with human serum. Production of interferon-g was inhibited by cocultured media with MSCs while MSCs also induced a significant inhibition of cell cycle in T cells.

DISCUSSION
In conclusion, PL or autologous serum could offer an alternative to the use of FCS in MSC expansion for clinical use maintaining the same growing potential, phenotype, immunomodulatory properties, and differentiation potential.

CITA DEL ARTÍCULO  Transfusion. 2009 Sep;49(9):1901-10. Epub 2009 May 20

tal vezLE INTERESE

¿QUÉ TECNOLOGÍA UTILIZAMOS?

La Clínica es el hospital privado con mayor dotación tecnológica de España, todo en un único centro.

Imagen de un PET, tecnología de vanguardia en la Clínica Universidad de Navarra

NUESTROS
PROFESIONALES

Los profesionales de la Clínica realizan una labor continuada de investigación y formación, siempre en beneficio del paciente.

Imagen profesionales de la Clínica Universidad de Navarra

POR QUÉ VENIR
A LA CLÍNICA

Conozca por qué somos diferentes a otros centros sanitarios. Calidad, rapidez, comodidad y resultados.

Imagen del edificio de la Clínica Universidad de Navarra