Scientific publications

Development and validation of a sensitive method for the determination of ganciclovir in human plasma samples by reversed-phase high-performance liquid chromatography

Campanero MA, Sadaba B [SP], García-Quetglas E, Azanza JR [SP].
Department of Clinical Pharmacology, University Clinical of Navarra, University of Navarra, Pamplona, Spain.

Magazine: Journal of Chromatology

Date: Sep 1, 1998

Clinical Research Unit [SP] Clinical Pharmacology [SP]

ABSTRACT

A rapid, sensitive, specific liquid chromatographic method has been developed for the determination of therapeutic levels of ganciclovir in human plasma.

Plasma (1 ml) and acyclovir (I.S.) were treated with 50% trichloroacetic acid. The supernatant was neutralized with 2 M NaOH and purified with chloroform. The aqueous phase (80 microl) was analyzed by a 3-microm Hypersil ODS C18 column with 0.04 M triethylamine-0.1 M sodium dihydrogen phosphate monohydrate as the mobile phase (1 ml/min) and ultraviolet detection at 254 nm.

Calibration was linear from 50 to 10000 ng/ml. Intra- and inter-day C.V. did no exceed 6.65%. The detection limit was about 10 ng/ml.

CITATION  J Chromatogr B Biomed Sci Appl. 1998 Mar 20;706(2):311-7

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