Scientific publications

Rapid liquid chromatographic assay for the determination of acetaminophen in plasma after propacetamol administration: application to pharmacokinetic studies

Campanero MA, Calahorra B, García-Quétglas E, López-Ocáriz A, Honorato J.
Servicio de Farmacología Clínica, Clinica Universitaria de Navarra, Universidad de Navarra, Pamplona, Spain.

Magazine: Journal of Pharmaceutical and Biomedical Analysis

Date: Jun 1, 1999

Clinical Pharmacology [SP]


A simple method for the rapid estimation of acetaminophen in plasma is described here. p-Propionamidophenol was used as internal standard.

The assay involved a single ethyl acetate extraction and liquid chromatographic analysis at a wavelength of 242 nm using a reversed-phase encapped column, with a mobile phase of acetonitrile and 0.005 M potassium dihydrogen phosphate adjusted at pH 3.00. The limit of quantitation of acetaminophen by this method was 0.05 microg ml(-1), only 0.1 ml of the plasma sample was required for the determination. The calibration graph was linear from 0.05 to 100 microg ml(-1). Intra and inter-day precision (CV) did not exceed 8.93%. Mean recoveries of 90.31% with a CV of 1.38% were obtained.

Applicability of the method was demonstrated by a pharmacokinetic study in normal volunteers who received 2 mg propacetamol.

CITATION  J Pharm Biomed Anal. 1999 Jun;20(1-2):327-34

you maybe interested


The Clínica is the greater private hospital with technological equipment of Spain, all in a single center.

Imagen de un PET, tecnología de vanguardia en la Clínica Universidad de Navarra


The professionals of the Clínica perform continuous research and training, always to the benefit of the patient.

Imagen profesionales de la Clínica Universidad de Navarra


Learn why we are different from other healthcare centers. Quality, speed, comfort and results.

Imagen del edificio de la Clínica Universidad de Navarra