Scientific publications

Pre- and postprandial expression of the leptin receptor splice variants OB-Ra and OB-Rb in murine peripheral tissues

Frühbeck G [SP]., Gómez-Ambrosi J. [SP], Martínez J.A.
Department of Physiology and Nutrition, University of Navarra, Pamplona, Spain

Magazine: Physiological Research

Date: Mar 1, 1999

Endocrinology and Nutrition [SP]

Leptin receptors (OB-R) are widely distributed in peripheral tissues. However, the RT-PCR data published on the distribution of OB-R are not always consistent.

The present study was undertaken in order to test whether the different muscle fiber type profile or the acute nutritional status in which tissue samples were excised from animals may influence OB-R expression. Six 12-week-old male Swiss-Webster mice were killed by decapitation either 1 h after feeding or after a 16-h fast, and the kidneys, testes, brown adipose tissue, gastrocnemius (G), soleus (SOL) and extensor digitorum longus (EDL) muscles were dissected out. In parallel, muscle fibers obtained from other animals were classified on the basis of differences in the staining intensity for myofibrillar adenosinetriphosphatase. The expression of OB-R isoforms was assessed by RT-PCR and ethidium bromide staining.

The signal for OB-Ra and OB-Rb was detected in all tissues examined. No differences were observed in samples obtained from either fed or fasted mice. G, SOL and EDL muscles showed the same pattern of OB-R expression.

Neither the short-term nutritional changes of the animal as regards to the pre- versus the postprandial-state nor differences in muscle fiber type had any influence on the qualitative expression of the OB-R splice variants a and b in the murine tissues studied. However, quantitative differences cannot be ruled out.

CITATION  Physiol Res. 1999;48(3):189-95

you maybe interested

WHAT TECHNOLOGY
DO WE USE?

The Clínica is the greater private hospital with technological equipment of Spain, all in a single center.

Imagen de un PET, tecnología de vanguardia en la Clínica Universidad de Navarra

OUR
PROFESSIONALS

The professionals of the Clínica perform continuous research and training, always to the benefit of the patient.

Imagen profesionales de la Clínica Universidad de Navarra

WHY CHOOSE
THE CLINICA?

Learn why we are different from other healthcare centers. Quality, speed, comfort and results.

Imagen del edificio de la Clínica Universidad de Navarra