Scientific publications

Modulation of beta 2-adrenoceptors in human lymphocytes by in vitro stimulation with mediators

Sanz ML [SP], de la Cuesta CG, Oehling A.
Department of Allergology and Clinical Immunology, School of Medicine, University of Navarra, Pamplona, Spain

Magazine: Journal of Investigational Allergology and Clinical Immunology

Date: Jun 1, 1994

Allergology and Immunology Department

Since Szentivanyi established the beta-adrenergic theory, many attempts have been made to explain the dynamics of bronchial hyperreactivity and cytotropic allergic reaction by this mechanism. In previous work, we found that beta-adrenoceptor numbers were decreased in symptomatic patients and after specific antigenic exposure.

As a result, we postulated that this might be due to mediator activity. Blood was extracted from 46 healthy donors for this study. Radioligand [125I]-iodocyanopindolol was used for beta-adrenoceptor determination in peripheral blood lymphocytes following Brodde's method.

Student's t test was used for statistical analysis. beta-Adrenoceptor number was determined before and after exposure to mediators (histamine, PAF, LTD4), and after melittin (phospholipase A2, PLA2, activator) exposure. A significant decrease was obtained after histamine (p < 0.05), LTD4 (p < 0.05) and melittin (p < 0.001) exposure; PAF did not modify the number of beta-adrenoceptors.

In conclusion, our results confirm that down-regulation of beta-adrenoceptors can be induced by mediator release.

CITATION  J Investig Allergol Clin Immunol. 1994 May-Jun;4(3):116-21

you maybe interested


The Clínica is the greater private hospital with technological equipment of Spain, all in a single center.

Imagen de un PET, tecnología de vanguardia en la Clínica Universidad de Navarra


The professionals of the Clínica perform continuous research and training, always to the benefit of the patient.

Imagen profesionales de la Clínica Universidad de Navarra


Learn why we are different from other healthcare centers. Quality, speed, comfort and results.

Imagen del edificio de la Clínica Universidad de Navarra