Modulation of beta 2-adrenoceptors in human lymphocytes by in vitro stimulation with mediators
Sanz ML [SP], de la Cuesta CG, Oehling A.
Department of Allergology and Clinical Immunology, School of Medicine, University of Navarra, Pamplona, Spain
Magazine: Journal of Investigational Allergology and Clinical Immunology
Date: Jun 1, 1994Allergology and Immunology Department
Since Szentivanyi established the beta-adrenergic theory, many attempts have been made to explain the dynamics of bronchial hyperreactivity and cytotropic allergic reaction by this mechanism. In previous work, we found that beta-adrenoceptor numbers were decreased in symptomatic patients and after specific antigenic exposure.
As a result, we postulated that this might be due to mediator activity. Blood was extracted from 46 healthy donors for this study. Radioligand [125I]-iodocyanopindolol was used for beta-adrenoceptor determination in peripheral blood lymphocytes following Brodde's method.
Student's t test was used for statistical analysis. beta-Adrenoceptor number was determined before and after exposure to mediators (histamine, PAF, LTD4), and after melittin (phospholipase A2, PLA2, activator) exposure. A significant decrease was obtained after histamine (p < 0.05), LTD4 (p < 0.05) and melittin (p < 0.001) exposure; PAF did not modify the number of beta-adrenoceptors.
In conclusion, our results confirm that down-regulation of beta-adrenoceptors can be induced by mediator release.
CITATION J Investig Allergol Clin Immunol. 1994 May-Jun;4(3):116-21
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