Scientific publications

MicroRNA signatures of iPSCs and endoderm-derived tissues

Porciuncula A, Zapata N, Guruceaga E, Agirre X, Barajas M, Prosper F.
Hematology and Cell Therapy Area, Clínica Universidad de Navarra, Avda. Pio XII 36, 31008 Pamplona, Spain; Division of Oncology, Center for Applied Medical Research (CIMA), University of Navarra, Avda. Pio XII 55, Pamplona 31008, Spain

Magazine: Gene Expression Patterns

Date: Sep 8, 2012

Cell Therapy Area [SP]

ABSTRACT

MicroRNAs (miRNAs), small non-coding RNAs that fine-tune gene expression, play multiple roles in the cell, including cell fate specification. We have analyzed the differential expression of miRNAs during fibroblast reprogramming into induced pluripotent stem cells (iPSCs) and endoderm induction from iPSCs upon treatment with high concentrations of Activin-A.

The reprogrammed iPSCs assumed an embryonic stem cell (ESC)-like miRNA signature, marked by the induction of pluripotency clusters miR-290-295 and miR-302/367 and conversely the downregulation of the let-7 family. On the other hand, endoderm induction in iPSCs resulted in the upregulation of 13 miRNAs. Given that the liver and the pancreas are common derivatives of the endoderm, analysis of the expression of these 13 upregulated miRNAs in hepatocytes and pancreatic islets revealed a tendency for these miRNAs to be expressed more in pancreatic islets than in hepatocytes.

These observations provide insights into how differentiation may be guided more efficiently towards the endoderm and further into the liver or pancreas. Moreover, we also report novel miRNAs enriched for each of the cell types analyzed.

CITATION   2012 Sep 8. pii: S1567-133X(12)00074-9. doi: 10.1016/j.gep.2012.08.002

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