Scientific publications

Evaluation of bioluminescent imaging for noninvasive monitoring of colorectal cancer progression in the liver and its response to immunogene therapy

Maider Zabala (1,4), Pilar Alzuguren (1), Carolina Benavides (2), Julien Crettaz (1), Gloria Gonzalez-Aseguinolaza (1), Carlos Ortiz de Solorzano (2), Manuela Gonzalez-Aparicio (1), Maria Gabriela Kramer (1,5), Jesus Prieto (3) and Ruben Hernandez-Alcoceba (1)
(1) Division of Gene Therapy and Hepatology. CIMA, University of Navarra. Foundation for Applied Medical Research. Av. Pio XII.Pamplona, Spain
(2) Morphology and Imaging Unit. CIMA, University of Navarra. Foundation for Applied Medical Research. Av. Pio XII. Pamplona,Spain
(3) CIBERehd. University Clinic of Navarra. Pamplona, Spain
(4) Institute for Stem Cell Biology and Regenerative Medicine, University of Stanford, 1050 Arastradero Road, Palo Alto, CA, USA
(5) Peter MacCallum Cancer Research Institute, Cancer Immunology Program, St Andrews Place, East Melbourne, 3001 Australia

Magazine: Molecular Cancer

Date: Jan 7, 2009

Hepatology

BACKGROUND
Bioluminescent imaging (BLI) is based on the detection of light emitted by living cells expressing a luciferase gene. Stable transfection of luciferase in cancer cells and their inoculation into permissive animals allows the noninvasive monitorization of tumor progression inside internal organs. We have applied this technology for the development of a murine model of colorectal cancer involving the liver, with the aim of improving the pre-clinical evaluation of new anticancer therapies.

RESULTS
A murine colon cancer cell line stably transfected with the luciferase gene (MC38Luc1) retains tumorigenicity in immunocompetent C57BL/6 animals. Intrahepatic inoculation of MC38Luc1 causes progressive liver infiltration that can be monitored by BLI. Compared with ultrasonography (US), BLI is more sensitive, but accurate estimation of tumor mass is impaired in advanced stages. We applied BLI to evaluate the efficacy of an immunogene therapy approach based on the liver-specific expression of the proinflammatory cytokine interleukin-12 (IL-12). Individualized quantification of light emission was able to determine the extent and duration of antitumor responses and to predict long-term disease-free survival.

CONCLUSION
We show that BLI is a rapid, convenient and safe technique for the individual monitorization of tumor progression in the liver. Evaluation of experimental treatments with complex mechanisms of action such as immunotherapy is possible using this technology.

CITATION  Mol Cancer. 2009 Jan 7;8:2

you mayBE INTERESTED

WHAT TECHNOLOGY
DO WE USE?

The Clínica is the greater private hospital with technological equipment of Spain, all in a single center.

Imagen de un PET, tecnología de vanguardia en la Clínica Universidad de Navarra

OUR
PROFESSIONALS

The professionals of the Clínica perform continuous research and training, always to the benefit of the patient.

Imagen profesionales de la Clínica Universidad de Navarra

WHY CHOOSE
THE CLINICA?

Learn why we are different from other healthcare centers. Quality, speed, comfort and results.

Imagen del edificio de la Clínica Universidad de Navarra