Scientific publications

Depot-specific differences in the lipolytic effect of leptin on isolated white adipocytes

Frühbeck G [SP], Gómez-Ambrosi J [SP].
Laboratory of Metabolic Research, Department of Endocrinology, Clínica Universitaria de Navarra, 31008 Pamplona, Spain.

Magazine: Medical Science Monitor

Date: Feb 1, 2002

Endocrinology and Nutrition [SP]

BACKGROUND
In the present study we hypothesized that leptin-induced lipolytic effects show depot-specific differences.

MATERIAL/METHODS
Three-month-old male lean (+/+) and obese fa/fa rats were used. Lipolysis was determined in the absence or presence of leptin (0.63, 6.25 and 62.5 nM) together with agents acting at the A1 adenosine receptor of fat cells isolated from both subcutaneous and omental depots. The glycerol released into the incubation medium was taken as the lipolytic rate index.

RESULTS
The highest dose of leptin produced a 57.5 +/- 7.3% and 70.3 +/- 3.8% increase in omental and subcutaneous glycerol release, respectively, compared to the basal lipolytic rate (P<0.001). The addition of the three leptin concentrations in the ligand-free state produced a significantly greater stimulation of lipolysis in subcutaneous fat cells (P=0.0331; P=0.0003 and P=0.0015, respectively) compared to omental adipocytes. Under adenosine A1 receptor agonism and antagonism the same pattern of response was observed between visceral and subcutaneous adipocytes of lean rats. Although adenosine deaminase produced near maximum lipolysis in the adipocytes of lean animals, only half of the maximum lipolytic rate (50.9 +/- 3.2%) was achieved in fat cells from fa/fa rats (P=0.0034). Leptin had no effect on the lipolytic activity of adipocytes from either localization in fa/fa rats.

CONCLUSIONS
It can be concluded that decreased sensitivity to the lipolytic effect of leptin in omental adipocytes compared to subcutaneous fat cells may underlie, at least in part, the association of visceral fat accumulation with increased co-morbidities.

CITATION  Med Sci Monit. 2002 Feb;8(2):BR47-55

you mayBE INTERESTED

WHAT TECHNOLOGY
DO WE USE?

The Clínica is the greater private hospital with technological equipment of Spain, all in a single center.

Imagen de un PET, tecnología de vanguardia en la Clínica Universidad de Navarra

OUR
PROFESSIONALS

The professionals of the Clínica perform continuous research and training, always to the benefit of the patient.

Imagen profesionales de la Clínica Universidad de Navarra

WHY CHOOSE
THE CLINICA?

Learn why we are different from other healthcare centers. Quality, speed, comfort and results.

Imagen del edificio de la Clínica Universidad de Navarra