A sensitive method for the determination of gemfibrozil in human plasma samples by RP-LC
González-Peñas E, Agarraberes S, López-Ocariz A, García-Quetglas E, Campanero MA, Carballal JJ, Honorato J.
Departamento de Química Orgánica y Farmacéutica, Facultad de Farmacia, Universidad de Navarra, 31008 Pamplona, Spain.
Magazine: Journal of Pharmaceutical and Biomedical Analysis
Date: Aug 1, 2001Clinical Pharmacology [SP]
A sensitive high-performance liquid chromatographic assay for the quantitative determination of gemfibrozil is described in this work. Ibuprofen was used as internal standard. The assay involved a single cyclohexane extraction and LC analysis with fluorescence detection. Chromatography was performed at 40 degrees C on a Hypersil ODS column. The mobile phase was a mixture of a solution of phosphoric acid 0.4% and acetonitrile (45:55). The method was validated.
The detection limit of this method was 0.025 microg ml(-1); only 0.5 ml of the plasma sample was required for the determination. The calibration graph was linear from 0.05 to 0.5 microg ml(-1) and required a cubic equation from 0.5 to 30 microg ml(-1). Intra and inter-day precision (C.V.) did no exceed 15%.
Mean recoveries were of 90.15+/-6.9% (C.V.'s<8%) for gemfibrozil and 93.10% for ibuprofen Applicability of the method was demonstrated by a pharmacokinetic study in normal volunteers who received gemfibrozil by oral route.
CITATION J Pharm Biomed Anal. 2001 Aug;26(1):7-14
you mayBE INTERESTED
The Clínica is the greater private hospital with technological equipment of Spain, all in a single center.
The professionals of the Clínica perform continuous research and training, always to the benefit of the patient.
Learn why we are different from other healthcare centers. Quality, speed, comfort and results.