A comparison between percutaneous and surgical transplantation of autologous skeletal myoblasts in a swine model of chronic myocardial infarction
Juan José Gavira (a), Maitane Pérez-Ilzarbe (b), Gloria Abizanda (b), Alba García-Rodríguez (e), Josune Orbe (d), José Antonio Páramo (b,d), Miriam Belzunce (d), Gregorio Rábago (a), Joaquín Barba (a), Jesús Herreros (a), Ángel Panizo (c), José A. García de Jalón (e), Diego Martínez-Caro (a) and Felipe Prósper (b,d)
(a) Department of Cardiology and Cardiovascular Surgery, University of Navarra, Spain
(b) Hematology and Cell Therapy, University of Navarra, Spain
(c) Department of Pathology, Clínica Universitaria, University of Navarra, Spain
(d) Foundation for Applied Medical Research, Division of Cancer and Division of Physiology, University of Navarra, Spain
(e) Departament of Animal Pathology, Veterinary Faculty, University of Zaragoza, Spain
Magazine: Cardiovascular Research
Date: Sep 1, 2006Haematology and Hameotherapy Cardiac Surgery [SP] Cardiology Pathological Anatomy [SP] Cell Therapy Area [SP]
Our aim was to compare the efficacy of surgical versus percutaneous administration of skeletal myoblasts (SkM) in a swine model of chronic myocardial infarction and to determine the mechanism(s) involved in their beneficial effect.
Two months after induction of myocardial infarction (MI), Goettingen miniature pigs underwent autologous SkM transplant either by direct surgical injection (n=6) or percutaneous access and intramyocardial delivery under fluoroscopic and echocardiographic guidance (n=6). Control animals received media alone (n=4). Functional analysis was performed by 2D echocardiography. Myoblast engraftment, in vivo cell differentiation, vessel formation, fibrosis, and the ratio between collagen type I/III deposition were analyzed in the infarct (IA) and non-infarct area (NIA) by immunohistochemistry.
Animals received a median of 407.55±115x106 BrdU-labeled autologous SkM. Myoblast transplant was associated with a statistically significant increase in left ventricular ejection fraction (p<0.01), increased vasculogenesis and decreased fibrosis (p<0.05), and reduced collagen type I/III ratio in the IA and NIA areas as compared with control animals. No differences were found between groups receiving SkM by percutaneous or surgical access.
Our results indicate that increased vasculogenesis and changes in matrix remodeling with decreased fibrosis are associated with the beneficial effect of SkM transplant in chronic MI. The equivalent benefit observed from surgical and percutaneous delivery has important clinical implications.
CITATION Cardiovasc Res. 2006 Sep 1;71(4):744-53
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